Determination of the Kinetic Rate Constants and Affinity of a Protein-Antibody Interaction Using the BiOptix 404pi™
Biophysical characterization of antigen-antibody interactions is an important application for
label-free instrumentation. In a previous report, Katsamba et al. (1) used the binding of
prostate-specific antigen (PSA) to a monoclonal antibody (mAb) to characterize antibody-antigen
interactions using surface plasmon resonance technology. In this application note, we used the
same model system to demonstrate that the BiOptix 404pi yields similar results.