SPR Without Compromises

When purchasing laboratory instruments you usually have to choose between cost and some measure of quality. The 404pi Enhanced Surface Plasmon Resonance System exists to break that norm. It offers scientists that require label-free, real-time detection of biomolecular interactions an extremely high sensitivity, low noise instrument at a price point that is hundreds of thousands of dollars less than systems with equivalent performance. The 404pi concurrently offers industry-leading reliability, easy to use software for experimental design and instrument control, and greater experimental flexibility.


Unrivalled Performance per Dollar

With sensitivity down to 100 Da, an extremely low noise profile, and an affordable price, no other SPR instrument comes close to giving you as much performance per dollar as the 404pi. You can finally get industry-leading SPR performance without the exorbitant price tag.

Built for Reliability

Most SPR flow cells are prone to clogging and require regular replacement – often twice per year. These kinds of flow cells are not covered under warranty and cost many thousands of dollars to replace. The 404pi’s fluidics cartridge is designed to prevent blockages and can be readily unclogged if necessary. We’re so confident that our fluidics cartridges will live a long and fruitful life, we warranty them for two years.

Your 404pi will not only have a lower operating cost than other SPR systems, but you’ll have less downtime as well. You can even run more viscous and complex solutions, such as human serum, without fear of clogging.

Features of the BiOptix 404pi include:

  • Phase-based SPR for high sensitivity
  • 4 injector / 4 flow cell design for increased throughput, allowing for multiplexed kinetic measurements
  • Increased uptime – unique fluidic system – no annual replacement required
  • Standard & custom surface chemistries
  • Advanced fluidic system for fast kinetic measurements
  • In-line degassing
  • Supplied with industry standard Scrubber 2 software
  • Cost effective and simple to operate

Biological Applications:

  • Protein-small molecule interactions
  • Protein-protein interactions
  • Antibody-antigen interactions
  • Kinetics & affinities
  • Affinity maturation
  • Epitope binning
  • Nucleic acid-protein interactions
  • Biomolecule concentration measurements
  • Immunogenicity screening

Enhanced Throughput & Flexibility

The BiOptix 404pi utilizes a four needle autosampler and four parallel flow cells. This allows operation in a mode that provides four times the throughput of an ordinary SPR instrument, while maintaining the sensitivity that users demand. The 404pi can also be operated in a high resolution mode for more detailed analysis of molecular interactions.

Making SPR Easier than Ever

The 404pi is easier to use than any other SPR system, especially for users who are not already experts in SPR. Our software for instrument control and experimental design has a simple, intuitive, drag & drop interface. The 404pi’s software can be used while the instrument is in operation, allowing one user to design an experiment while another is running an experiment. Less user maintenance is required because of our ultra-reliable flow cell design.

SPR is all we do, so every member of our support team is a dedicated SPR expert. We’re happy to help with instrument operation, assay development, and data analysis! We know that obtaining a complete understanding of your scientific objective is important. We try to make it simple, too.

404pi Data

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