Label-free Multiplex Detection of MicroRNAs
MicroRNAs (miRNAs) have gained considerable attention as potentially predictive biomarkers for a number of disorders. Mounting evidence suggests that miRNAs are differentially expressed in a variety of cancers and thus have potential for early detection, prognosis, and monitoring of response to treatment (Calin et al. 2006). Because of their small size and low concentrations, miRNAs in biological fluids are demanding targets for detection, requiring advanced diagnostic technologies. In this application note, protocols are described that allow for the specific detection of picomolar concentrations of cancer related miRNAs by utilizing the BiOptix 404Pi™ in combination with a signal enhancing monoclonal antibody that recognizes DNA-RNA hybrid duplexes (Hu et al. 2006).